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联系人:袁先生

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同位素标记培养基

同位素标记培养基

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产品描述

(1) ISOGRO™-13C Powder - Growth Medium 99%atom C-13 

(2) ISOGRO™-D Powder - Growth Medium 97-99%atom D

(3) ISOGRO™-15N Powder - Growth Medium 98%atom N-15

(4) ISOGRO™-13C, N Powder - Growth Medium 99%atom C-13; 98%atom N-15

(5) ISOGRO™-15N, D Powder-Growth Medium,98%atom N-15; 97-99%atom D

(6) ISOGRO™-13C,15N,D Powder - Growth Medium 99%atom C-13; 98%atom N-15; 97-99%atom D

使用配制ISOGRO™粉末配制培养液的步骤

准备100ml ISOGRO™培养液:

1. 放1克 ISOGRO™粉末在90毫升Millipore® 水

2. 使用以下浓度的盐类溶液在培养液中:

K2HPO4 100 g/L 1.8 mL

KH2PO4 50 g/L 2.8 mL

MgSO4 50 g/L 2.0 mL

CaCl2•H2O 37 g/L 30 μL

3.使用NaOH将PH值调整到7并将溶液的体积用Millipore® 水加至100ml.

4. Pass the solution through a 0.22 μm filter and transfer the filtrate

to an autoclaved shaker flask (for example:

50 mL medium in a 500 mL flask).

5. The culture is inoculated with a loop of E. coli which has been

maintained on a nutrient agar slant.

6. Shake the culture flask in a 37 °C water bath.

7. The absorbance of the culture is measured at 600 nm with a

1:3 dilution into water.

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